Premium
Group 5 determination in Pooideae grass pollen extracts by monoclonal antibody‐based ELISA. Correlation with biologic activity
Author(s) -
Ramírez J.,
Obispo T. M.,
Duffort O.,
Carpizo J. A.,
Chamorro M. J.,
Barber D.,
Ipsen H.,
Carreira J.,
Lombardero M.
Publication year - 1997
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1997.tb02151.x
Subject(s) - pollen , allergen , monoclonal antibody , immunoelectrophoresis , chromatography , chemistry , potency , antibody , biology , immunology , botany , in vitro , biochemistry , allergy
A solid‐phase, monoclonal antibody‐based ELISA was set up to quantitale group 5 allergens in pollen extracts of wild and cultivated Pooideae grasses. The method was able to evaluate group 5 concentration in mass units with a sensitivity in the ng/ml range and a practical working range of 1–100 ng/ml. The group 5 ELISA was compared with rocket immunoelectrophoresis for determination of allergen levels in several Phleumpratense extracts, and a very good quantitative correlation was found ( r =0.98; P <0.0001). A highly significant correlation ( r >0.8) was also obtained in comparing allergenic potency determined by RAST inhibition to group 5 content in several wild and cultivated grass species. The results proved the usefulness of the method in the standardization of Pooideae pollen extracts employed in diagnosis and treatment.