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Detection of mosquito saliva‐specific IgE antibodies by capture ELISA
Author(s) -
BrummerKorvenkontio H.,
Palosuo K.,
Palosuo T.,
BrummerKorvenkontio M.,
Leinikki P.,
Reunala T.
Publication year - 1997
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1997.tb01002.x
Subject(s) - saliva , immunoglobulin e , antibody , immunology , medicine , biology
We developed an IgE‐capture ELISA and measured mosquito saliva‐specific IgE antibodies in 27 children sensitive to mosquito bites. Children with large 15‐min bite wheals had significantly higher ( P < 0.0005) mosquito saliva‐specific IgE levels than children with small wheals. In the latter group, the saliva‐specific IgE level was significantly higher ( P =0.031) than the levels of six infants never exposed to mosquitoes. A positive correlation ( r =0.65; P =0.0002) was found between the size of the 15‐min wheal and the mosquito saliva‐specific IgE antibody levels. These results further support the role of mosquito saliva‐specific IgE antibodies in the pathogenesis of mosquito‐bite whealing. Compared to immunoblotting, IgE‐capture ELISA provides a quantitative method to measure mosquito saliva‐specific IgE antibodies.