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Immunodominance of seven regions of a major allergen, Cry j 2, of Japanese cedar pollen for T‐cell immunity
Author(s) -
Hashiguchi S.,
Hino K.,
Taniguchi Y.,
Kurimoto M.,
Fukuda K.,
Ohyama M.,
Fujiyoshi T.,
Sonoda S.,
Nishimura Y.,
Yamada G.,
Sugimura K.
Publication year - 1996
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1996.tb04682.x
Subject(s) - immunodominance , immunology , allergen , biology , pollen , immunity , human leukocyte antigen , antibody , t cell , immune system , allergy , antigen , botany
The immunodominant regions of the Japanese cedar pollen allergen Cry j 2 for T‐cell immunity were determined with whole peripheral blood lymphocytes (PBL) derived from seven allergic patients and three nonallergic subjects. Cry j 2‐stimulated T‐cell proliferation was inhibited by anti‐HLA‐DR. but not by anti‐HLA‐DQ antibody, indicating that the responding T cells recognized the allergen peptides associated with HLA‐DR molecules. It was found that seven regions of Cry j 2, i.e., regions corresponding to amino acid numbers 1–26, 70–84, 151–167. 187–203, 252–279, 283–314, and 345–362, were immunodominant for T‐cell proliferation. Thus, Cry j 2 bears a limited number of immunodominant regions despite polymorphic features of HLA‐DR in the immune system. This suggests the possibility of molecularly designing Cry j 2 antagonists that could downregulate allergic reactions to Japanese cedar pollen.