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Enhancement of basophil histamine release by interleukinl‐3: reduced effect in atopic subjects
Author(s) -
Miadonna A.,
Salmaso C.,
Cottini M.,
Milazzo N.,
Tedeschi A.
Publication year - 1996
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1996.tb04664.x
Subject(s) - histamine , basophil , immunoglobulin e , allergy , immunology , medicine , stimulation , endocrinology , antibody
The inducing and enhancing effects of interleukin‐3 (IL‐3) on basophil histamine release in patients with respiratory allergy ( n = 28) and in normal subjects ( n = 22) were compared. Leukocyte suspensions. prepared by dextran sedimentation, were stimulated with anti‐IgE (1/5000), N ‐formyl‐methionyl‐leucyl‐phenylalanine (FMLP, 1 μM), and IL‐3 (0.1–10 ngiml), and histamine concentration was measured by an automated fluorometric method. A trend toward highef histamine release after challenge with anti‐IgE, FMLP, and IL‐3 was found in atopic subjects. Preincubation of basophils with IL‐3 resulted in a dose‐dependent increase of anti‐IgE‐ and FMLP‐induced histamine release, with a more marked effect in nonatopic than in atopic subjects. Mean net enhancement of anti‐IgE‐induced histamine release by 10 ng/ml IL‐3 was 2.5±5% in atopic subjects and 29.6±4.2% in nonatopic subjects ( P< 0.001). The enhancement of FMLP‐induced histamine release by IL‐3 was 10.3 ± 3.9% in atopic patients and 29±2.4% in nonatopic subjects ( P<0.01 ). In atopic subjects, a negative correlation was found between anti‐IgE‐ or FMLP‐induced histamine release and net enhancement by IL‐3 (r= ‐0.45, P<0.02; r= ‐0.48, P<0.01, respectively). The results of this study indicate that in atopic subjects IgE‐mediated histamine release can scarcely be enhanced by a basophil response modifier such as IL‐3. It is conceivable that the frequent basophil stimulation in atopic patients leads to a reduced sensitivity to the enhancing effect of IL‐3.