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Interleukin‐4 and interferon‐gamma production by peripheral bloGd mononuclear cells from food‐allergic patients
Author(s) -
André F.,
Pène J.,
André C.
Publication year - 1996
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1996.tb04623.x
Subject(s) - peripheral blood mononuclear cell , food allergy , immunology , medicine , allergy , cytokine , immunoglobulin e , interferon gamma , secretion , stimulation , interleukin 4 , interleukin 2 , interleukin , in vitro , biology , antibody , biochemistry
The aim of this study was to investigate whether patients with food allergy had a cytokine imbalance of interleukin (1L)‐4 and interferon‐gamma (IFN‐γ) production. Diagnostic procedures including skin prick tests, determination of food‐specific serum IgEs, and positive double‐blind, placebo‐controlled, food challenges identified 15 adult patients. They were compared with 15 age‐ and sex‐matched healthy subjects. Peripheral blood mononuclear cells (PBMC) were incubated for 24, 48, and 72 h in the presence of phytohemagglutinin plus phorbol myristate acetate. After mitogen stimulation, culture supernatants from patients with food allergy contained significantly less IFN‐γ but increased IL‐4 when compared with healthy controls. Secretion of IL‐4 was maximal at 24 h and IFN‐γ secretion was maximal at 72 h. There was no correlation between cytokine secretion in vitro and serum IgE level. These findings demonstrated that an imbalance of IL‐4 and IFN‐γ production is present in food allergy, as documented in other allergic diseases, but other mechanisms are probably also involved.