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ELISA method for detection of mite allergens in barn dust: comparison with mite counts
Author(s) -
Härfast B.,
Johansson E.,
Johansson S. G. O.,
HageHamsten M.
Publication year - 1996
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1996.tb04602.x
Subject(s) - mite , acaridae , acari , tyrophagus putrescentiae , biology , pyroglyphidae , barn , veterinary medicine , allergen , acariformes , house dust mite , botany , allergy , immunology , medicine , geography , archaeology
ELISA (enzyme‐linked immunosorbent assay) inhibition with a monoclonal antibody (mAb) (42B6) to Lepidoglyphus destructor was used to detect and quantify the storage‐mite allergens in 30 dust samples collected from barns. Regarding the mite fauna, microscopic inspection of the barn dust and mite counts showed that L. destructor infested all 30 barns investigated (range 430–195400 mites/g dust). In 29/30 barns, L. destructor constituted more than 70% of the Astigmata species. Acarus siro was found in 26 samples, the highest value being 16 155 nites/g. No Dermatophagoides species were found. As to mites of the suborder of Prostigmata, species belonging to seven different families were detected. Besides the predominant L. destructor , allergens derived from other storage mites such as Glycyphagus domesticus, A. siro , and Tyrophagus putrescentiue have previously been assessed by this ELISA method. The correlation between number of mites and concentrations of mite antigen as measured by ELISA was assessed by linear regression ( r 2 = 0.83). Thus, inhibition of mAb 42B6 in ELISA would seem to offer a simple and reliable method to detect levels of dust‐mite species belonging to the Acaridae and Glycyphagidae families.