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Cross‐reactivity of IgE‐binding components between boiled Atlantic shrimp and German cockroach
Author(s) -
Crespo J. F.,
Pascual C.,
Helm R.,
SanchezPastor S.,
Ojeda I.,
Romualdo L.,
MartinEsteban M.,
Ojeda J. A.
Publication year - 1995
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1995.tb02499.x
Subject(s) - german cockroach , cockroach , shrimp , immunoglobulin e , allergen , immunoassay , cross reactivity , western blot , biology , allergy , immunology , chemistry , antibody , biochemistry , cross reactions , fishery , ecology , gene
IgE‐antibody reactivity to boiled Atlantic shrimp ( Pandalus borealis ) and German cockroach ( Blattella germanica ) of sera from 89 patients, sensitive to one or the other, was investigated with an enzymatic immunoassay for specific IgE detection (CAP‐FEIA System, Pharmacia, Sweden). IgE serum levels to both antishrimp and anticockroach allergens were found to be positive in 76 of the 89 (85.4%) tested sera. A positive anticockroach IgE was very rare in the absence of detectable antishrimp IgE (five of 89 sera). Linear regression analysis on antishrimp and anti‐German cockroach IgE levels‐log plot revealed correlation coefficient (r) of 0.73. Inhibition experiments showed that boiled Atlantic shrimp extract inhibited CAP with German cockroach, and vice versa. Immunoblotting showed the strongest IgE binding for both allergenic extracts between 30 and 43 kDa. By blot inhibition, the binding capacity of German cockroach was totally‐abolished by Atlantic shrimp extract, while German cockroach extract only partially IgE binding to Atlantic shrimp. Cross‐reactivity exists between shrimp, an important food allergen, and German cockroach, which has an increasing role in allergic asthma. It could be important to determine the clinical significance of cross‐allergy to both allergens, in which exposures occur in different ways.