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The surface membrane antigen phenotype of human blood basophils
Author(s) -
Füreder W.,
Agis H.,
Sperr W. R.,
Lechner K.,
Valent P.
Publication year - 1994
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1994.tb00788.x
Subject(s) - microbiology and biotechnology , antigen , monoclonal antibody , cd38 , chemistry , membrane glycoproteins , transferrin receptor , biology , immunology , receptor , glycoprotein , biochemistry , antibody , cd34 , stem cell
Basophils are effector cells of allergic reactions and express a unique profile of cellular antigens (Ag). Using a combined toluidine‐blue/immunofluorescence staining method, we were able to study the cell membrane Ag phenotype of normal human blood basophils with monoclonal antibodies (mAbs) against established and novel CD antigens. According to previous findings, basophils express CD9 (p24), CDlla (LFA‐1 alpha‐chain), CDllb (C3biR), CDllc (CR4), CD13 (aminopeptidase N), CDwl7 (lactosylceramide), CD18 (beta‐chain of β 2 ), CD25 (IL‐2R alpha‐chain), CD26 (dipeptidylpeptidase), CD31 (PECAM), CD35 (CR1), CD38 (T10), CD43 (leukosialin), CD44 (Pgp‐1), CD45 (pan‐leukocyte Ag), and CD63 (basophil activation Ag). Various novel CD Ags were detected on basophils, including membrane cofactor protein (MCP) (CD46), the N‐linked glycan CD47, decay‐accelerating factor (DAF) (CD55), membrane attack complex inhibitory factor (MACIF) (CD59), LFA‐3 (CD58), ICAM‐2 (CD 102), ICAM‐3 (CD50), C5a receptor (CD88), MIC‐2/E2 (CD99), and the interleukin‐1 (IL‐1) R type II (CD121b). These data provide further evidence that basophils express a unique profile of surface membrane receptors for cytokines and immunomodulating compounds, as well as adhesion molecules and surface glycolipids.