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Cytokine modulation of basophil histamine release in wasp‐venom allergy
Author(s) -
Radermecker M.,
Louis R.,
Leclercq M.,
Weber Th.,
Corhay J. L.,
Bury Th.
Publication year - 1994
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1994.tb00133.x
Subject(s) - histamine , basophil , venom , immunology , allergy , cytokine , mast cell , immunoglobulin e , chemistry , biology , pharmacology , antibody , biochemistry
We report the effect of interleukin‐3 (IL‐3) and of other cytokines on antigen‐induced basophil histamine release in wasp‐venom‐allergic subjects. Leukocytes from 12 patients with documented anaphylactic sensitivity to wasp venom were preincubated in the presence or absence of IL‐3, granulocyte/macrophage‐colony stimulating factor (GM‐CSF), IL‐5, IL‐8, or stem cell factor (SCF). Washed cells were then exposed to venom and to other secretagogues, and histamine release in the supernatant was measured fluorometrically. Preincubation of leukocytes with IL‐3, GM‐CSF, or IL‐5 (0.02–2 ng/ml), but not with IL‐8 and SCF, caused a dose‐dependent enhancement of antigen‐induced basophilic histamine release in all subjects tested. Mean maximum increase was about 100% for IL‐3, IL‐5, and GM‐CSF. The priming effect of IL‐3 was rapid, persisted up to 12 h, and was not accompanied by a change in cellular histamine. IL‐3 had a comparable enhancing effect when basophils were triggered with anti‐IgE or N ‐formylmethionylphenylalanine (FMP). By contrast, IL‐3 had no effect on substance‐P‐induced histamine release. The significant enhancement of basophil releasability to antigen in wasp‐venom allergy by very low concentrations of IL‐3, GM‐CSF, and IL‐5 suggests that cytokines in the basophil (mast‐cell?) microenvironment could be critical factors in determining the variability of sting reactions in Hymenoptera‐venom‐allergic subjects.