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Does guanine concentration in house‐dust samples reflect house‐dust mite exposure?
Author(s) -
Hallas T.E.,
Yi Xue,
Schou C.
Publication year - 1993
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1993.tb02396.x
Subject(s) - guanine , mite , xanthine , pyroglyphidae , allergen , house dust mite , chemistry , dust mites , acari , toxicology , biology , immunology , biochemistry , allergy , zoology , botany , nucleotide , enzyme , gene
Using specific ELISA extracts, we analyzed samples of pure mite cultures and house dust for group I allergens from Dermatophagoides pteronyssinus , D. farinae , and D. microceras . Correspondingly, we measured the concentration of guanine and xanthine by rigorous chemical methods. The pure mite cultures were used to correiate allergen levels with guanine levels. The house‐dust samples one‐sidedly contained more guanine than expected. This suggests that considerable amounts of guanine originate from non‐house‐dust mite sources and implies that guanine quantifications are of limited clinical value.