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Basophil histamine release induced by anti‐IgE and concanavalin A Relation to the total plasma IgE content
Author(s) -
Nielsen B. Windelborg,
Hansen B.,
Damsgaard T. M. E.,
Herlin T.,
Søderberg U.,
Bjerke T.,
ThestrupPedersen K.,
SchiØtz P. O.
Publication year - 1993
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1993.tb02175.x
Subject(s) - immunoglobulin e , basophil , histamine , concanavalin a , immunology , chemistry , population , receptor , cord blood , antibody , endocrinology , biology , medicine , biochemistry , in vitro , environmental health
The plant lectin concanavalin A (Con A) has been shown to induce basophil histamine release by an IgE‐receptor‐dependent process resembling that of anti‐IgE‐antibodies. In this study, the concentration‐response for basophil histamine release from washed blood cells was analyzed in a population of blood samples from adults with a total plasma IgE content ranging from < 5 kU/l to > 18000 kU/l ( n = 108), as well as 148 cord blood samples. The concentration‐response‐curves for anti‐IgE in all adult blood samples were similar, despite the large variation in total plasma IgE ‐ only the cord blood samples showed a decreased sensitivity. In contrast, the optimal concentration of Con A was inversely related to plasma IgE, and this relation was most pronounced in the adult blood samples. It is proposed that IgE‐receptor‐mediated histamine release may be dependent not only on the number of stimulatory, dimeric cross‐links formed between IgE‐receptors, but also on the molecular structure of the cross‐linking agent.