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Immunoglobulin‐G‐dependent stimulation of guinea pig lung mast cells and macrophages
Author(s) -
AlLaith* M.,
Weyer A.,
Havet N.,
Dumarey C.,
Vargaftig B. B.,
Bachelet M.
Publication year - 1993
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1993.tb00757.x
Subject(s) - ovalbumin , histamine , immunoglobulin e , guinea pig , immunology , stimulation , mast cell , antibody , lung , antigen , medicine , biology , endocrinology
Alveolar macrophages and mast cells isolated from guinea pig lung were passively sensitized with IgG1, IgG2, or serum obtained from guinea pigs actively sensitized with ovalbumin. The release of histamine by mast cells and of thromboxane A2 by alveolar macrophages upon ovalbumin challenge indicated that both antibodies and serum were capable of sensitizing these cells with similar effectiveness. Heating the scrum at 56°C for 4 h to inactivate IgE did not modify the antigen‐dependent response of lung cells. These results suggest a predominant role for IgG in the allergic response of the guinea pig through the activation of different cell types such as lung mast cells and alveolar macrophages.