Premium
Characterization and partial purification of the Croatian national standard Dermatophagoides pteronyssinus allergen extract
Author(s) -
Tresščcec A.,
Kolevska T.,
Cvorišsšcec B.,
Krnić B.,
StipićMarković A.,
Tudman Z.,
Dekaris D.
Publication year - 1993
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1993.tb00744.x
Subject(s) - allergen , chromatography , sephadex , chemistry , immunoelectrophoresis , medicine , immunology , antibody , biochemistry , allergy , enzyme
Lyophilized Dermatophagoides pteronyssinus ( Der p ) allergen extract (AE) and partially purified Der p extract (PAE) Were Prepared and characterized. Partial purification of AE was performed by gel filtration on Sephadex G‐1OO and Sephacryl S‐300. Crossed immunoelectrophoresis (CIE) disclosed the same precipitating lines in AE and PAE preparations. The relative potencies of AE and PAE were determined and compared with the WHO International Standard for Der p by the RAST inhibition method. The potencies were 6.5 × 10 5 IU and 1.5 × 10 6 IU, respectively. Biologic standardization by quantitative skin testing was performed with AE (20 selected patients) and PAE (12 patients). Median C h was calculated by linear regression analysis (log‐log model). One ampoule of AE contained 65 300 BU and 1 ml (vial) of PAE contained 166000 BU. Der p AE could serve as a Croatian national standard for further production of Der p allergenic extracts.