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Occurrence of IgE antibodies against the 39‐kDa allergen component of the mite Lepidoglyphus destructor in urban and rural subjects
Author(s) -
HageHamsten M.,
Lagging E.,
Härfast B.,
Johansson S. G. O.
Publication year - 1993
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1993.tb00716.x
Subject(s) - immunoglobulin e , allergen , immunology , mite , antibody , medicine , allergy , chemistry , biology , ecology
Several species of storage mite have been shown to cause IgE‐mediated sensitization. Monoclonal antibodies (mabs) have been raised against the storage mite Lepidoglyphus destructor , and one mab (42B6) immunoprecipitated a band of 39 kDa of L. destructor . To search for the frequency of IgE antibodies in patient sera against this allergen component, we modified a radioimmunoassay (RIA), 42B6 being used as capturing antibody. Twenty of 43 sera from farmers who were RAST positive to L. destructor (46.5%) were scored as positive in this assay. There was a moderate degree of correlation between positive RAST results to L. destructor and the 39‐kDa allergen. Among 14 sera from Stockholm residents positive in RAST to both Dermatophagoides pteronyssinus and L. destructor , six detected the 39‐kDa allergen component. However, three sera from urban subjects lacking IgE antibodies against both mite species also had IgE antibodies against the 39‐kDa allergen. The present study demonstrates that the 39‐kDa allergen of L. destructor is an important allergen, and that rural as well as urban subjects can become sensitized.