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Allergens from rye pollen ( Secale cereale )
Author(s) -
Montero M. T.,
Alonso E.,
Sainz T.
Publication year - 1992
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1992.tb02245.x
Subject(s) - secale , pollen , allergen , chromatography , elution , chemistry , biology , botany , microbiology and biotechnology , allergy , immunology
Rye pollen was incubated for 30 min and proteins extracted at this time were collected as extract A (EA). The same pollen grains were resuspended in buffer and incubated for 18.5 h. Proteins extracted in this period were designated extract B (EB), Both extracts were subfractionated by DEAE ion‐exchange chromatography and allergen presence in peaks detected by the dot‐immnunobinding technique. The results reveal that unretained proteins (peaks 1 and 2) and proteins eluted at 0.2 M NaCl from extract B contain the highest proportion of allergens, SDS‐PAGE of chromatographic peaks showed that peak 2 from extract B contains a highly purified 28 kDa band. On the skin of allergic patients this band gave a stronger positive prick test than for the crude extract.