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Regulation of histamine release from human basophil leucocytes: role of H 1 , H 2 and H 3 receptors
Author(s) -
Tedeschi A.,
Lorini M.,
Arquati M.,
Miadonna A.
Publication year - 1991
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1991.tb00634.x
Subject(s) - histamine , thioperamide , histamine h3 receptor , histamine h2 receptor , basophil , histamine h1 receptor , histamine h4 receptor , receptor , histamine receptor , chemistry , medicine , endocrinology , cimetidine , agonist , metiamide , histamine n methyltransferase , immunoglobulin e , pharmacology , antagonist , biology , immunology , biochemistry , antibody
A novel class of histamine receptors (H 3 , controlling histamine synthesis and release, was described in rat and human brain and peripheral nerve endings. The present study was undertaken to evaluate whether H 3 , receptors contribute to the regulation of histamine release from human basophils. Basophil leucocytes were incubated with a H., antagonist (thioperamide; concentrations ranging from 1 nM to 10 μM) or with a H 3 agonist ((R)αmethyl‐histamine; concentrations ranging from I to 100 μM), and subsequently were stimulated with optimal doses of anti‐IgE and formyl‐methionyl‐leueyl‐phenyl‐alanine (f‐met peptide). No significant modifications of histamine release were observed after incubation either with the H 3 agonist or with the H 3 antagonist. By contrast, a H 3 antagonist (cimetidine; concentrations ranging from 1 to 100 μM) exerted a dose‐dependent enhancing effect on anti‐IgE‐ and, to a lesser extent, on f‐met peptide‐induced histamine release. A H, antihistamine (chlorpheniramine; concentrations ranging from 100 nM to 1μM), at the highest concentration employed, displayed an inhibitory activity on IgE‐dependent and IgE‐independent histamine release. Exogenous histamine was shown to exert a dose‐dependent inhibitory effect on two‐staged anti‐IgE‐induced histamine release. Taken as a whole, these results suggest that H 3 receptors are not involved in the regulation of histamine release from human basophils; by contrast, H 2 receptors participate in controlling histamine release from human basophils, as previously demonstrated by other authors.