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Existence of exine‐free airborne allergen particles of Japanese cedar ( Cryptomeria japonica ) pollen
Author(s) -
Takahashi Y.,
Sakaguchi M.,
Inouye S.,
Miyazawa H.,
Imaoka K.,
Katagiri S.
Publication year - 1991
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1991.tb00628.x
Subject(s) - cryptomeria , japonica , pollen , allergen , geology , medicine , botany , allergy , biology , immunology
We investigated whether exine‐free pollen allergen particles exist together with the intact pollen grains of Japanese cedar ( Cryptomeria japonica ) in the air during the pollen season in Yamagata City. First, we separated the allergen particles in an Andersen multi‐stage air‐sampler according to their aerodynamic diameters. The amount of major allergen ( Cry j I) on each stage of the sampler was determined by a sensitive fluorometric sandwich ELISA, and the pollen count of the same samples was done by light microscopy after Carberla staining. Cry j I was found in stages 1 to 6, whereas most of intact and ru|3tured pollen grains were microscopically observed only in stages 1 and 2. Second, we suctioned the air through a tandem membrane filter system (the first filter, Nuclepore filter with 5 um‐pores; and the second, Millipore filter with 0.3 μm‐pores). None of the pollen grains was detectable on the 0.3 μm‐pore filter with light microscopy. However, Cry j I was detectable in the aqueous extract from the second filter. From these results, we concluded that pollen‐free Cry j I existed in the air of Yamagata City during the pollen season.