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Histamine measurement in whole blood and cell supernatants
Author(s) -
Rotz B.,
Savaser A.,
Werthmann I,
Lau S.,
Wahn U.
Publication year - 1991
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1991.tb00616.x
Subject(s) - histamine , radioimmunoassay , chemistry , basophil , whole blood , immunoglobulin e , in vitro , antibody , chromatography , monoclonal antibody , blood cell , immunology , biochemistry , pharmacology , medicine
Histamine as an important mediator of the allergic reaction has been measured by a number of methods both in whole blood and cell supernatants after in vitro release. We compared the fluorometric histamine assay with two radioimmunoassays and studied detection limit, recovery, cross‐reactivity and accuracy in buffer‐based standards as well as the sensitivity after IgE‐mediated in vitro release from washed cells and whole blood. Our data indicate that the double‐antibody RIA (Pharmacia) can be used for studies of basophil histamine release in both whole blood and cell supernatants. Due to significant cross‐reactivity to N‐methyl‐histamine it cannot be used for histamine determinations in plasma. The second radioimmunoassay using succinyl‐glycin as an acylating reagent and monoclonal antibodies, which have been raised to acylated histamine, is the most sensitive assay without any cross‐reactivity with methylhistamine.