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Aeroallergen analyses and their clinical relevance
Author(s) -
Johnsen C.R.,
Abrahamsen L.,
Skov P. STAHL,
Johansen N.,
Poulsen L.K.
Publication year - 1991
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1991.tb00611.x
Subject(s) - allergen , aeroallergen , immunoglobulin e , chromatography , chemistry , immunology , radioallergosorbent test , serial dilution , allergy , medicine , antibody , pathology , alternative medicine
The aim was to compare IgE and IgG 4 , RAST‐inhibition assay (RI), monoclonal antibody ELISA (Mab‐ELISA), counter current immuno electrophoresis (CCIE) and histamine release from basophil leukocytes (HR) for allegen quantification with special reference to aeroallergen detection. As components of indoor acroallergens, cat, dog, and Derm. pter. allergen extracts were selected for the experiments. To evaluate unspecific interference, these allergens were compared mutually and with Cladosporium herbarum. Allergen extracts in varying dilutions were mixed with crushed glass fibre filter materials, eluted, recovered by centrifugation, and allergen concentration quantified by the assays. Equal sensitivity was found for both IgE‐ and IgG 4 ‐RI assaying eat allergen (in the range 5 − 50 SQ‐U/ml) and dog allergen (in the range 10 2 − 10 3 SQ‐U/ml). The IgG 4 ‐RI assaying Derm. pter , was more sensitive (50 SQ‐U/ml) than IgE‐RI (2*10 3 SQ‐U/ml). The ranges of allergen detection limits for the Mab‐ELISA were equal for cat and Derm. pter. (10 – 10 2 SQ‐U/ml). The range of allergen detection limits for CCIE, assaying dog were 10 4 − 10 5 SQ‐U/ml. The ranges of allergen detection limits for HR were equal for eat and Derm. pter. (10 – 10 2 SQ‐U/ml), and 10 2 − 10 3 SQ‐U/ML for dog. Because of cross‐reactivity, a minor degree of interference was observed in the IgE‐RI and the HR test for the highest concentration of cat and dog allergens.