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Naturally occurring aliphatic polyamines‐induced histamine release from rat peritoneal mast cells
Author(s) -
Kurosawa M.,
Uno D.,
Kobayashi S.
Publication year - 1991
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1991.tb00597.x
Subject(s) - histamine , spermine , spermidine , polyamine , chemistry , mast cell , putrescine , biochemistry , immunoglobulin e , pharmacology , microbiology and biotechnology , immunology , biology , enzyme , antibody
Rat peritoneal mast cells were incubated with different concentrations of naturally occurring aliphatic polyamines, spermine and spermidine, at 0.1‐10mM and the amount of histamine release into the supernatant solutions was measured. The addition of each polyamine to the suspensions of the mast cells caused a histamine release in a dose‐dependent manner. The effect of 10 mM spermine and spermidine was as much as that of 0.5 μg/ml compound 48/80. The histamine release from the cells incubated with each polyamine was rapid and the amount of histamine release into the supernatant solutions reached a maximum at 1 min with the incubations. 0.1 mM spermine, which in itself could not cause a significant histamine release, showed a tendency to enhance anti‐IgE‐induced histamine release from the mast cells.