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Maxisorp
Author(s) -
Poulsen L. K.,
Pedersen M. F.,
Malling H.J.,
Søndergaard I.,
Weeke B.
Publication year - 1989
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1989.tb02258.x
Subject(s) - radioallergosorbent test , allergen , immunoglobulin e , isotype , immunology , aeroallergen , chemistry , allergy , antibody , biology , monoclonal antibody
For determination of allergen‐specific IgE in cell culture supernatants and other highly diluted IgE preparations a radioallergosorbent test (RAST) based on high adsorption polystyrene test tubes has been developed (“Maxisorp RAST”). Cladosporium herbarum extract was used as a model allergen but timothy grass pollen, house dust mite and dog dander showed similar results. The test showed specificities of both allergen and immunoglobulin isotype and significant correlations ( r = 0.67–0.88) with established RAST procedures were found. Based on immunosorbent‐purified allergen‐specific IgE the estimated sensitivity was within the order of 150‐300 pg allergen‐specific IgE per ml. The within‐assay variation was 4‐9% and the inter‐assay‐variation 17–29%. The Maxisorp RAST is useful as an inhibition assay for quantitating allergenic activity down to 0.1 biological units/ml of allergen extracts.