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In vitro studies of degradation of birch and timothy pollen allergen preparations by human duodenal juice
Author(s) -
Einarsson R.,
Renck B.,
Taudorf E.
Publication year - 1988
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1988.tb00921.x
Subject(s) - allergen , hydrolysis , pollen , in vitro , protease , chemistry , food science , biochemistry , allergy , enzyme , botany , biology , immunology
The hydrolysis of birch and timothy pollen allergen preparations by duodenal juice from an adult volunteer was studied in vitro with the aid of rocketimmunoelectrophoresis, radiorocketimmunoelectrophoresis and RAST‐ inhibition. The results achieved with all methods were similar. The duodenal juice had the capacity to hydrolyse both the grass and tree pollen proteins, and after 30 min preincubation only 10 % of the original birch and timothy allergenic activity remained. Heat treatment of the duodenal juice completely destroyed the proteolytic activity (i.e. no hydrolysis occurred) while protease inhibitors only marginally prevented the hydrolysis.