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Effect of interleukin 2 on basophil histamine release
Author(s) -
Morita Y.,
Goto M.,
Miyamoto T.
Publication year - 1987
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1987.tb02367.x
Subject(s) - histamine , basophil , chemistry , immunoglobulin e , pharmacology , interleukin , endocrinology , medicine , immunology , cytokine , antibody
Accumulating evidence suggests a link between immediate hypersensitivity and cellular immunity. In this study, we examined the effect of interleukin 2 (IL‐2) on basophil histamine release. Histamine‐releasing activity of IL‐2 was very weak with % histamine release of 2.9 ± 1.3 (mean ± SEM, n = 9) at 1:12 dilution. IL‐2 at 1:1200 dilution slightly inhibited anti‐IgE‐induced histamine release by 22.4 ± 18.6% ( P > 0.05). There was a significant potentiation of release at 1:12 dilution of IL‐2 with % enhancement of 78.7 ± 42.2 ( P < 0.05). IL‐2 enhanced the calcium ionophore A23187‐induced histamine release in a dose‐dependent fashion. IL‐2 at 1:12 dilution significantly potentiated release by 28.8 ± 6.3% ( P < 0.05). There was a slight suppression of formyl‐methionyl‐leucyl‐phenylalanine (FMLP)‐induced histamine release at 1:1200 dilution with % inhibition of 23.4 ± 7 4 ( P > 0.05). At 1:12 dilution, IL‐2 significantly potentiated FMLP‐induced release by 73.7 ± 41.6% ( P < 0.05). Recombinant IL‐2 (RIL‐2) augmented anti‐IgE‐induced histamine release with a significant enhancement at 200 units/ml. Conventional IL‐2 was more potent than RIL‐2 in enhancing release. These results indicate that IL‐2 enhances basophil histamine release and some part of the effect of IL‐2 on basophils is derived from other factors contained in conventional IL‐2.