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Inhibition of T cell‐dependent antibody production by D‐penicillamine
Author(s) -
Petersen J.
Publication year - 1987
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1987.tb02185.x
Subject(s) - pokeweed mitogen , phytohaemagglutinin , polyclonal antibodies , penicillamine , peripheral blood mononuclear cell , antibody , antigen , microbiology and biotechnology , in vitro , cytokine , biology , immunology , chemistry , lymphocyte , medicine , biochemistry
The effects of D‐penicillamine (D‐Pen) on the production of immunoglobulins (Ig) and anti‐microbial antibodies (Ab) by human mononuclear cells (MNC) cultured in vitro were analysed by haemolytic plaque forming cell (PFC) assays. Polyclonal Ig and Ab production, induced by pokeweed mitogen (PWM), was not affected by D‐Pen in pharmacologically relevant concentrations, unless Cu 2+ , was added. Likewise, D‐Pen + Cu 2+ , but not D‐Pen alone, affected polyclonal Ig production induced by Epstein‐Barr virus. The production of interleukin‐2 and B cell growth factor by phytohaemagglutinin‐stimulated T cells was not inhibited by D‐Pen; again D‐Pen + Cu 2+ markedly reduced the production of these cytokines. By contrast, D‐Pen significantly reduced the antigen‐induced antibody production without requirement of additional Cu 2+ . Addition of cytokine‐containing supernatant to MNC treated with D‐Pen + Cu 2+ tended to increase the PWM‐induced Ig responses, but had no effect on the antigen‐induced Ab production of MNC cultured with D‐Pen. Thus, the mechanisms by which D‐Pen suppresses polyclonal and antigen‐induced B lymphocyte responses are different.