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Humoral Immunity to Dietary Antigens in Atopic Dermatitis
Author(s) -
Husby S.,
Schultz Larsen F.,
Hyltoft Petersen P.,
Svehag SE.
Publication year - 1986
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1986.tb00316.x
Subject(s) - atopic dermatitis , immunology , ovalbumin , antigen , antibody , medicine , humoral immunity , immunoglobulin e , allergy , avidin , biotin , biology , biochemistry
Enzyme‐linked immunosorbent assays (ELISA) employing a biotin‐avidin amplification step are described for the quantification of human serum IgG antibodies to the dietary antigens ovalbumin (QA) and beta‐lactoglobulin (BLG). The analytical quality of these assays was acceptable. Antibodies were measured in 16 patients with mild or moderate atopic dermatitis (AD), in 31 patients with a history of AD, and in closely matched controls. Levels of serum anti‐QA antibodies did not differ in patients and controls, whereas anti‐BLG antibodies tended to be higher in patients with mild or moderate AD than in controls (P < 0.05).