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Characterization of IgE and IgG Antibody Responses to Dermatophagoides farinae in Rats
Author(s) -
NORDVALL S. L.,
DALE S.,
BJÖRKSTÉN B.
Publication year - 1985
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1985.tb04148.x
Subject(s) - immunoglobulin e , immunology , antibody , alum , antigen , allergen , ovalbumin , adjuvant , radioallergosorbent test , cyclophosphamide , antibody response , chemistry , biology , allergy , medicine , chemotherapy , organic chemistry
Rats (Brown Norway/Wistar Fu) that were pretreated or not pretreated with cyclophos‐phamide were immunized with varying amounts of Dermatophagoides farinae allergen extract with alum as adjuvant. Sera were analysed by RAST and by crossed radioimmunoelectro‐phoresis (CRIE). The highest IgE antibody responses were recorded in animals pretreated with cyclophosphamide that had received low immunizing doses of antigen. The IgE antibody pattern in CRIE was, however, not influenced by the dose of antigen or by cyclophosphamide pretreatment. The IgG pattern in CRIE closely resembled the IgE pattern, demonstrating a similar specificity of the antibody responses of the two isotypes. This indicates non‐class specific control of the specificity of antibody responses.