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A Mononuclear Cell‐Derived Histamine Releasing Factor in Asthmatic Patients
Author(s) -
Alam R.,
Rozniecki J.
Publication year - 1985
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1985.tb02671.x
Subject(s) - histamine , phytohaemagglutinin , bronchoconstriction , immunology , provocation test , in vivo , lymphokine , allergen , medicine , peripheral blood mononuclear cell , in vitro , allergy , asthma , lymphocyte , antigen , chemistry , biology , pathology , biochemistry , alternative medicine , microbiology and biotechnology
In an attempt to study the role of lymphokines in asthma, lymphocytes from asthmatic patients were stimulated in vitro with skin test‐positive allergen (dust mite) or with non‐specific mitogen‐phytohaemagglutinin for 4h, then washed carefully and cultured alone for 16h. Cell‐free supernatants were subsequently collected and applied in the basophil histamine release test in vitro and in bronchial provocation test and skin prick test in vivo . Supernatant of non‐stimulated lymphocytes from asthmatic patients released significant amounts of histamine from basophils. Stimulation of lymphocytes with specific allergen or phytohamagglutinin augmented the supernatant‐induced histamine release. Lymphocyte supernatants from the majority of patients also induced bronchoconstriction and elicited skin wheal and flare reaction in vivo .