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Reproducibility of Histamine Skin Prick Test
Author(s) -
Taudorf E.,
Malling H.J.,
Laursen L. C.,
Lanner Å.,
Weeke B.
Publication year - 1985
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1985.tb00245.x
Subject(s) - histamine , reproducibility , coefficient of variation , medicine , skin test , anesthesia , pharmacology , chemistry , chromatography , pathology , tuberculosis
The reproducibility of skin prick test using histamine dihydrochloride 1, 5, and 10 mg/ml was tested by three nurses in five non‐atopics in a double‐blind trial. The variations day‐to‐day, within‐day, between and for the same tester were calculated. Seventy‐five percent of wheal reactions obtained by histamine 1 mg/ml were < 15 mm 2 . With histamine 5 mg/ml there were only a few wheals < 15 mm 2 and none at all with bistamine 10 mg/ml. The mean coefficient of variation of wheals > 15 mm2 was between 20–30%, in contrast to figures between 30‐60% with wheals < 15 mm2. No significant day‐to‐day or within‐day variation was shown concerning histamine wheal areas. It is suggested that histamine dihydrochloride 10 mg/ml should replace histamine dihydrochloride 1 mg/ml as the positive reference in routine skin prick tests and biological standardization.