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ELISA in Diagnosis of Respiratory Allergy
Author(s) -
Negrini A. C.,
Troise C.,
Voltolini S.
Publication year - 1985
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1985.tb00227.x
Subject(s) - respiratory allergy , medicine , immunoglobulin e , allergy , asthma , radioallergosorbent test , immunology , intoxicative inhalant , respiratory system , allergen , antibody , biology , toxicology
An enzyme‐linked immunosorbent assay (ELISA), employing beta‐galactosidase conjugated anti‐IgE, was used for the determination of specific IgE antibodies to common inhalant allergens ( Dermatophagoides pt. and f. , Parietaria and four grass pollens) in serum samples from 82 adult patients with asthma and/or rhinitis. A total of 194 analysis were carried out and the results were compared with those of skin tests and RAST. The correlation coefficient ( r ) between ELISA and RAST results was high ( r = 0.95); the correlation between skin tests and ELISA ( r = 0.93) was greater than that between skin tests and RAST ( r = 0.90). No significant differences were found among the allergens used. We conclude that the version of ELISA used develops an overall good correlation with skin tests and RAST and seems to provide a satisfactory alternative to RAST for allergy diagnosis.