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Standardization of House Dust Mite Extracts Liberation of Allergenic and Antigenic Components in Relation to Extraction Conditions
Author(s) -
Kauffman H. F.,
Heide S.,
Hovenga H.,
Vries K.
Publication year - 1985
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1985.tb00209.x
Subject(s) - house dust mite , immunoglobulin e , extraction (chemistry) , mite , allergen , chemistry , chromatography , antigen , potency , liberation , pyroglyphidae , immunology , allergy , biology , biochemistry , antibody , in vitro , botany
The spontaneous release of house dust mite components from cultures of Dermatophagoides pteronyssinus into slightly buffered water was studied against time, using both continuous and discontinuous extraction procedures. It was shown that proteins, carbohydrates, IgE binding components and precipitating antigenic components were rapidly released from the house dust mite cultures, reaching a maximal liberation within 1 h of extraction. Repeated extractions of house dust mite cultures (discontinuous extraction) showed an additional release of IgE components but the IgE binding potency declined after successive extractions, while showing increasing release of immunological inactive components. IgE binding to antigens immobilized to polystyrene surfaces (IgE‐ELISA) appeared to be less sensitive compared with cyanogen‐bromide activated discs (IgE‐RAST). It was concluded that extraction procedures of house dust mite cultures with short incubation time of 1 h or less are to be preferred.