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Determination of Specific IgG Antibody by Crossed Radioimmunoelectrophoresis
Author(s) -
Nordvall S. L.,
Uhlin T.,
Einarsson R.
Publication year - 1983
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1983.tb01604.x
Subject(s) - antibody , immunology , immunoglobulin e , medicine , immunoglobulin g , radioallergosorbent test
A crossed radioimmunoelectrophoretic method was developed for detection of honey bee venom specific IgG antibodies in patient sera. At the serum concentration 1/200 the contrast between specific binding and background was the most favourable. The detection limit was fairly low, approximately 30 kU/1 (IgG RAST units). A reference system based on the reference kits in Phadebas IgG‐RAST® was elaborated.