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Quantitative Determination of 1,4‐Methyl‐Imidazoleacetic Acid in Urine by High Performance Liquid Chromatography
Author(s) -
Søndergaard Ib
Publication year - 1982
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1982.tb02343.x
Subject(s) - chromatography , urine , histamine , metabolite , atopic dermatitis , high performance liquid chromatography , chemistry , quantitative analysis (chemistry) , asthma , medicine , immunology , biochemistry
A reversed phase ion‐pair high performance liquid chromatographic (HPLC) method for quantitative determination of the major histamine metabolite 1,4‐methyl‐oleacetic acid (1,4‐MIAA) in urine is described. The sample handling is minimal as the sample only has to be centrifuged and diluted before analysis, in contrast to earlier gas chromatographic and thin‐layer chromatographic methods. The method correlates well with a thin‐layer chromatographic determination ( r =0.79, n =15), and results from analysis of urine from different patients suffering from mastocytosis, chronic urticaria, atopic dermatitis and asthma are presented together with samples from normal individuals. Most of the patients analysed showed increased excretion of 1,4‐MIAA compared with the controls. Future applications of the method, including analysis on patients with food allergy and patients with abnormalities in their histamine metabolism, are discussed.