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Biochemical and Allergenic Properties of the House Dust Mite Extract, Dermatophagoides pteronyssinus
Author(s) -
Ishii Akika,
Shimomura Hiroshi,
Hashiguchi Junichi,
Kabasawa Yasuhiro
Publication year - 1982
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1982.tb01912.x
Subject(s) - chemistry , uronic acid , chromatography , sialic acid , fractionation , house dust mite , allergen , mite , biochemistry , food science , polysaccharide , biology , allergy , botany , immunology
A Further study has been made on the house dust mite extract, Dermatophagoides pteronyssinus , with emphasis on gel‐filtrated fraction 2 (F 2 ). The crude mite extract showed at least nine discs on polyacrylamide disc electrophoresis and contained less than 0.25 % sialic acid and less than 0.5 mM hexosamine and no detectable uronic acid. From gel filtration of the crude extract a No. 2 fraction (F 2 ) with allergenic activity showed at least five components on SDS disc electrophoresis covering a molecular weight range of between 15,000 and 70,000. The major allergenic activity of F 2 dissolved in pH 7–8 and 4–5 on an isoelectric focusing column. Affinity chromatography of lectins showed that allergenic activity did not relate to structures of N ‐acetyl‐D‐glucosamine or N ‐acetyl‐D‐galactosamine. Allergenic activity of the crude extract was not affected by peptic digestion and the mite digest prepared by trypsin and pronase showed a similar fraction‐action and activity profile as crude extract.