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Crossed Radioimmunoelectrophoretic Studies of Bee Venom Allergens
Author(s) -
Aukrust L.,
Einarsson R.,
ÖHman S.,
Johansson S. G. O.
Publication year - 1982
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1982.tb01909.x
Subject(s) - bee venom , venom , allergen , medicine , allergy , immunology , dermatology , biology , zoology , ecology
Crossed immutioelectrophoresis (CIE) glass slides of boney bee venom showing 18 immunoprecipitates were used in crossed radioimmunoelectrophoresis (CRIE) experiments with sera from 25 patients allergic to honey bee venoms. Phospholipase A, acid phosphatase, hyaluronidase and melittin were demonstrated in the immunoprecipitates during zymograpbic techniques and direct hemolysis of sheep erythrocytes. The CRIE experiments verified the allergenicity of these proteins. In addition, another antigen (Ag‐1) with allergenic activity was identified. This allergen is probably identical to the previously described allergen C in honey bee venom. The presence and complexity of multiple forms of the known allergenic proteins in honey bee venom, was well illustrated in the CIE/CRIF system employed.