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Measurement of IgG Antibodies against Timothy Pollen Allergens
Author(s) -
Prahl P.,
Skov P,
Minuva U.,
Weeke B.
Publication year - 1981
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1981.tb04106.x
Subject(s) - antibody , antigen , blocking antibody , immunology , pollen , chemistry , immunoglobulin e , microbiology and biotechnology , biology , botany
This study describes a microtiter double antibody assay for the measurement of specific IgG, which is believed to be identical with blocking antibodies. The precipitates were harvested on glass fiber filters, and the activity from iodinated antigens fixed in the precipitates was used as a measure of the level of the serum blocking antibodies.