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Quantitation of Passive Cutaneous Anaphylaxis (PGA) by Using Radiolabelled Antigen
Author(s) -
Ring Johannes,
Seifert Jorgen,
Brindel Walter
Publication year - 1978
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1978.tb01523.x
Subject(s) - antiserum , serial dilution , antigen , chemistry , antibody , evans blue , anaphylaxis , saline , human serum albumin , microbiology and biotechnology , immunology , chromatography , medicine , pathology , allergy , biology , alternative medicine
The major problem of detecting reaginic antibody by passive cutaneous anaphylaxis IPCA) is the quantitation of the dye reaction. Radiolabelled antigen was used in an attempt to quantitate the PCA reaction (Radio‐PCA). Antisera containing reaginic antibody against human serum albumin (HSA) were produced in rabbits. These antisera were injected into normal rabbit skin in different dilutions. Twenty‐four hours later BSA was injected intravenously either with Evans Blue or as 125‐1‐HSA. Radioactivity found in antibody‐containing skin was significantly higher than in control specimens containing saline or normal rabbit serum, as low as antiserum dilutions of 1:1,000. Compared with the Evans Blue technique Radio‐PCA was able to distinguish quantitatively between different antiserum dilutions at a higher level of statistical significance.