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MIGRATION INHIBITION OF HUMAN LEUKOCYTES MIXED WITH PHYTOHEMAGGLUTININ‐PREINCUBATED MONONUCLEAR LEUKOCYTES
Author(s) -
CLAUSEN JENS ERIK
Publication year - 1975
Publication title -
allergy
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 3.363
H-Index - 173
eISSN - 1398-9995
pISSN - 0105-4538
DOI - 10.1111/j.1398-9995.1975.tb00200.x
Subject(s) - citation , medicine , peripheral blood mononuclear cell , university hospital , library science , family medicine , computer science , biology , genetics , in vitro
An in vitro test able to demonstrate phytohemagglutinin (PHA)-induced migration inhibition of human peripheral blood leukocytes was investigated. The migration inhibition was demonstrated by the agarose migration technique. Purified mononuclear leukocytes which had been incubated for 30 min with PHA, then washed and added to non-separated leukocytes induced migration inhibition of the mixed cell population. Since no PHA was added to the non-separated leukocytes, a direct PHA effect on polymorph migration was avoided. If the PHA-preincubated mononuclear leukocytes were heated at 56 degrees C for 30 min, their ability to cause migration inhibition was abolished. This suggests that the inhibition was caused by migration inhibitory factor (MIF) produced by the PHA-stimulated lymphocytes.