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Predicting Function from Structure: 3D Structure Studies of the Mammalian Golgi Complex
Author(s) -
Mogelsvang Soren,
Marsh Brad J.,
Ladinsky Mark S.,
Howell Kathryn E.
Publication year - 2004
Publication title -
traffic
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 2.677
H-Index - 130
eISSN - 1600-0854
pISSN - 1398-9219
DOI - 10.1111/j.1398-9219.2004.00186.x
Subject(s) - golgi apparatus , biology , microbiology and biotechnology , ceramide , sphingolipid , sphingomyelin , endosome , biochemistry , membrane , endoplasmic reticulum , intracellular , apoptosis
3D electron tomography studies of the structure of the mammalian Golgi complex have led to four functional predictions (1). The sorting and exit site from the Golgi comprises two or three distinct trans ‐cisternae (2). The docking of vesicular–tubular clusters at the cis ‐face and the fragmentation of trans ‐cisternae are coordinated (3). The mechanisms of transport through, and exit from, the Golgi vary with physiological state, and in different cells and tissues (4). Specialized trans ‐ER functions in the delivery of ceramide to sphingomyelin synthase in the trans ‐Golgi membrane, for the regulated sorting via sphingolipid‐cholesterol‐rich domains. These structure‐based predictions can now be tested using a variety of powerful cell and molecular tools.