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Decreased methylation of the NK3 receptor coding gene ( TACR3 ) after cocaine‐induced place preference in marmoset monkeys
Author(s) -
Barros Marilia,
Dempster Emma L.,
Illott Nicholas,
Chabrawi Soha,
Maior Rafael S.,
Tomaz Carlos,
De Souza Silva Maria A.,
Huston Joseph P.,
Mill Jonathan,
Müller Christian P.
Publication year - 2013
Publication title -
addiction biology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 1.445
H-Index - 78
eISSN - 1369-1600
pISSN - 1355-6215
DOI - 10.1111/j.1369-1600.2011.00409.x
Subject(s) - marmoset , conditioned place preference , cpg site , dna methylation , epigenetics , dopamine transporter , methylation , receptor , serotonin transporter , dopamine , biology , gene , pharmacology , serotonin , genetics , neuroscience , transporter , gene expression , paleontology
Epigenetic processes have been implicated in neuronal plasticity following repeated cocaine application. Here we measured DNA methylation at promoter CpG sites of the dopamine transporter ( DAT1 ) and serotonin transporter ( SERT ) and neurokinin3‐receptor (NK3‐R)‐receptor ( TACR3 ) coding genes in marmoset monkeys after repeated cocaine injections in a conditioned place preference paradigm. We found a decrease in DNA methylation at a specific CpG site in TACR3 , but not DAT1 or SERT . Thus, TACR3 is a locus for DNA methylation changes in response to repeated cocaine administration and its establishment as a reinforcer, in support of other evidence implicating the NK3‐R in reinforcement‐ and addiction‐related processes.