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A peptide carrier for the delivery of elastin into fibroblast cells
Author(s) -
Nasrollahi Saman Ahmad,
Fouladdel Shamileh,
Taghibiglou Changiz,
Azizi Ebrahim,
Farboud Effat Sadat
Publication year - 2012
Publication title -
international journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 93
eISSN - 1365-4632
pISSN - 0011-9059
DOI - 10.1111/j.1365-4632.2011.05214.x
Subject(s) - elastin , fibroblast , peptide , fluorescence microscope , biophysics , dermal fibroblast , biochemistry , microbiology and biotechnology , chemistry , medicine , biology , pathology , fluorescence , in vitro , physics , quantum mechanics
Background Transmembrane delivery of active peptides and proteins, including skin delivery of cosmeceutical proteins such as collagen and elastin, has been a challenging issue. Amphipathic cell‐penetrating peptides (CPPs) have been proposed as carrier peptides to mediate cellular uptake of proteins without covalent binding. Materials and methods In this study, we have used a short peptide, Pep‐1, as our CPP to transport elastin into fibroblast cells. Different ratios of Pep‐1/elastin complexes were produced by using a fixed amount of elastin and different molar ratio of Pep‐1. The ability of transduction into cells was determined by fluorescence microscopy. The characteristics of Pep‐1/elastin complexes were monitored using scanning electron microscopy and photon correlation spectroscopy. Results No cellular toxicity was observed in cells treated with Pep‐1/elastin complex. Finally, we determined a Pep‐1 : elastin ratio of 10 : 1 as the most effective ratio in cellular delivery of elastin. Conclusion Pep‐1 mediated fast and effective delivery of elastin as a cosmetic protein into fibroblast cells in the treatment of skin‐aging symptoms.