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Isolation of both Pseudozyma aphidis and Nocardia otitidiscaviarum from a mycetoma on the leg
Author(s) -
Chen Bin,
Zhu Linyu,
Xuan Xuan,
Wu Lingjian,
Zhou Tieli,
Zhang Xueqi,
Li Bingxu
Publication year - 2011
Publication title -
international journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 93
eISSN - 1365-4632
pISSN - 0011-9059
DOI - 10.1111/j.1365-4632.2010.04814.x
Subject(s) - mycetoma , nocardia , medicine , isolation (microbiology) , actinomycetales , nocardiosis , nocardiaceae , microbiology and biotechnology , dermatology , biology , genetics , bacteria , streptomyces
We describe a case of mycetoma which typified the classic presentation of the disease: a male farmer with affection of the lower limbs and a history of trauma. The patient presented with a swollen right lower limb showing multiple discharging sinuses for 25 years. Histopathologically, grains were found by HE stain, and clustered yeast‐like cells were observed by PAS stain. The distinctive ‘dot‐in‐circle’ sign was found through MRI. Besides Nocardia otitidiscaviarum , Pseudozyma aphidis was isolated from deep tissue culture, and the identification of the etiologic species was ascertained by DNA sequencing. Generally speaking, Nocardia otitidiscaviarum is an infrequent cause of mycetoma, and Pseudozyma species are usually isolated from plant material rather than clinical specimens. This is the first case of mycetoma from which both Nocardia otitidiscaviarum and Pseudozyma aphidis were isolated.