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Nicotine induces the expression of early growth response‐1 in human skin dermal fibroblasts
Author(s) -
Choi Jae Eun,
Kim Ji Na,
Jeong Sang Hoon,
Son Sang Wook
Publication year - 2010
Publication title -
international journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 93
eISSN - 1365-4632
pISSN - 0011-9059
DOI - 10.1111/j.1365-4632.2009.04210.x
Subject(s) - nicotine , reverse transcription polymerase chain reaction , messenger rna , cell growth , medicine , viability assay , real time polymerase chain reaction , human skin , microbiology and biotechnology , blot , cell , andrology , biology , biochemistry , gene , genetics
Background Egr‐1 is known to play an important role in cell growth, differentiation, and cell survival. Human skin dermal fibroblasts (HSDF) may be an important target for adverse effects of tobacco components. However, nicotinic effect for early growth response‐1 (Egr‐1) expression is unknown in HSDF. Methods Cytotoxicity of nicotine was assessed by cell viability test in HSDF. The expression of Egr‐1 protein and mRNA after nicotine treatment was evaluated by Western blotting and real‐time reverse transcription–polymerase chain reaction. We also measured the promoter activity of Egr‐1 in HSDF after nicotine exposure. Results Early growth response‐1 protein and mRNA levels were increased in dermal fibroblasts exposed to nicotine. Nicotine treatment stimulated the promoter activity of Egr‐1 in cultured human fibroblasts. Conclusion In this study, we demonstrate that Egr‐1 expression is markedly induced in HSDF after exposure to nicotine.