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The anti‐condyloma acuminatum effects of interferon‐inducible protein 10 in vitro
Author(s) -
Li Huijuan,
Yi Tao,
Zhao Shuyun,
Chen Pin,
Cheng Chenxian,
Wei Yuquan,
Zhao Xia
Publication year - 2009
Publication title -
international journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 93
eISSN - 1365-4632
pISSN - 0011-9059
DOI - 10.1111/j.1365-4632.2009.03776.x
Subject(s) - condyloma acuminatum , tunel assay , transfection , apoptosis , immunohistochemistry , terminal deoxynucleotidyl transferase , recombinant dna , microbiology and biotechnology , flow cytometry , in vitro , interferon , medicine , biology , virology , cell culture , immunology , gene , human papillomavirus , biochemistry , genetics
Background Interferon‐inducible protein 10 (IP‐10) has been reported to show an effective antiviral and antineoplastic role in various murine models. Aim To investigate the anti‐condyloma acuminatum effect and human papillomavirus (HPV)‐inhibiting efficacy of a recombinant plasmid encoding IP‐10 in vitro . Methods A recombinant plasmid DNA carrying IP‐10 cDNA was constructed. Condyloma acuminatum tissue particles were transfected with IP‐10 and examined for apoptosis by terminal deoxynucleotidyl transferase‐mediated dUTP nick end labeling (TUNEL) staining and flow cytometry techniques. Relative real‐time polymerase chain reaction (PCR) was used to validate the HPV inhibited level of the treatment groups. Immunohistochemistry was used to determine the IP‐10 expression on condyloma acuminatum tissues. Results Transfected IP‐10 was expressed mainly in the cytoplasm of the condyloma acuminatum tissues. Plentiful apoptosis was observed in condyloma acuminatum tissues transfected with IP‐10. In addition, HPV expression was lower in IP‐10‐treated tissues than in control tissues. Conclusions These observations suggest that IP‐10 has strong anti‐condyloma acuminatum effects, inducing apoptosis and inhibiting HPV, and therefore may be a novel and potentially effective therapy for condyloma acuminatum.