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Effect of γδ T cell supernatant on human skin fibroblast proliferation and collagen production – Possible role of transforming growth factor‐β and basic fibroblast growth factor
Author(s) -
Ohtsuka Tsutomu
Publication year - 2008
Publication title -
international journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 93
eISSN - 1365-4632
pISSN - 0011-9059
DOI - 10.1111/j.1365-4632.2008.03805.x
Subject(s) - fibroblast , cell growth , basic fibroblast growth factor , growth factor , t cell , microbiology and biotechnology , immunology , receptor , cell culture , medicine , biology , immune system , biochemistry , genetics
Background Perivascular mononuclear cell infiltration, vascular injury and diffuse interstitial fibrosis has been shown in systemic sclerosis (SSc). Objective To study a role of cellular immune mechanism inducing fibroblast proliferation and collagen synthesis. Methods The interaction of γδ T‐cell receptor bearing lymphocyte supernatant to human fibroblast proliferation and collagen synthesis was studied. Results The fibroblasts added with γδ T cell supernatant showed increased proliferation compared with those without it and that added with αβ supernatant. The endothelial cells added with γδ T cell supernatants, however, showed significantly decreased proliferation compared with those without it and that added with αβ T cell supernatant. The fibroblasts added with endothelial cell supernatant showed increased proliferation compared with those without it. A doubling of collagen synthesis was observed when human skin fibroblasts were maintained in all of the supernatant. The collagen synthesis was inhibited by antitransforming growth factor‐β antibody and antibasic fibroblast growth factor antibody. Conclusion γδ T–cell‐receptor bearing lymphocyte has proliferative and collagen synthesis activity to human skin fibroblasts, but suppressive to endothelial cells.