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Peripheral blood mononuclear cells proliferation and Th1/Th2 cytokine production in response to streptococcal M protein in psoriatic patients
Author(s) -
PérezLorenzo Rolando,
NúñezOreza Luis Alberto,
GarmaQuen Patricia Margarita,
LópezPacheco Eduardo,
BricaireBricaire Guadalupe
Publication year - 2006
Publication title -
international journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 93
eISSN - 1365-4632
pISSN - 0011-9059
DOI - 10.1111/j.1365-4632.2004.02528.x
Subject(s) - peripheral blood mononuclear cell , medicine , immunology , cytokine , antigen , streptococcus pyogenes , psoriasis , streptococcus , stimulation , immune system , staphylococcus aureus , biology , in vitro , bacteria , biochemistry , genetics
Background Psoriasis is a chronic skin disease that is probably a T cell‐mediated autoimmune condition which is strongly associated with streptococcal throat infections. Although some groups have associated the involved response with different streptococcal antigens, M protein has been described as the major virulence factor of Streptococcus pyogenes . Thus, it is necessary to describe some features of the cellular responses to this streptococcal antigen. Methods Proliferation and Th1/Th2 cytokine production of peripheral blood mononuclear cells (PBMC) in response to total soluble extracts from type M5 S. pyogenes with (TSE37Sp) and without (M − TSESp) M protein were analyzed in 10 psoriatic patients and 10 healthy controls. Results PBMC from both patients and controls proliferated to both extracts. Responses to M − TSESp were significantly lower than those to TSE37Sp ( P < 0.05). PBMC IL‐2 and γIFN production after TSE37Sp stimulus was much higher than after M − TSESp antigenic stimulation in both groups ( P < 0.05). Meanwhile, IL‐4 production was quite low in both groups and in response to both extracts. We found a differential production of IL‐10 between groups. PBMC from healthy controls responded to TSE37Sp with a much higher production of this cytokine as compared to the responses showed to M − TSESp while the cells from psoriatic patients responded without differences in the production of IL‐10. Conclusion Results obtained suggest an important Th1 response to M protein in psoriatic patients which could be associated with the cellular responses involved in psoriasis, while healthy subjects respond in a probably non‐Th2 IL‐10 producing regulatory T cells fashion.