Premium
Linear IgA dermatosis with IgA and IgG autoantibodies to the 180 kDa bullous pemphigoid antigen (BP180): evidence for a distinct subtype
Author(s) -
Metz Brandie J.,
Ruggeri Sun Young,
Hsu Sylvia,
Reed Jon A.,
Ghohestani Ali S.,
Uitto Jouni,
Ghohestani Reza F.
Publication year - 2004
Publication title -
international journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 93
eISSN - 1365-4632
pISSN - 0011-9059
DOI - 10.1111/j.1365-4632.2004.02016.x
Subject(s) - medicine , autoantibody , bullous pemphigoid , immunology , pemphigoid , antigen , antibody
Background Autoantibodies in linear immunoglobulin A (IgA) disease (LAD) are reported to be of IgA class and directed against a 97–120 kDa epidermal antigen. Methods We report a 39‐year‐old woman with clinical features of LAD and with circulating IgA and IgG autoantibodies to the 180 kDa bullous pemphigoid antigen (BP180). Results Histopathology of lesional skin revealed a subepidermal blister with mixed inflammatory cell infiltrate. Direct immunofluorescence of perilesional skin showed linear deposits of IgA along the dermal–epidermal junction. The antigen specificity of the patient's circulating antibodies was determined by Western blotting and enzyme‐linked immunoabsorbent assay (ELISA) using various antigen sources, including cultured human keratinocytes, dermal protein lysates, and purified laminin‐5, as well as proteins corresponding to BP180, the 230 kDa bullous pemphigoid antigen (BP230), laminin‐5 subunits, and collagen IV α1–α6 chains. IgA and IgG antibodies in the patient's serum were directed against BP180, and no IgA or IgG reactivity was found against the other skin antigens. Conclusions These data provide evidence for the presence of a subtype of LAD with dual IgA and IgG autoimmune response to BP180.