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IMMUNOHISTOLOGIC LOCALIZATION OF RAS P21 IN NORMAL, HYPERPLASTIC, AND NEOPLASTIC EPIDERMIS
Author(s) -
INOHARA SHINICHI,
KITANO YUKIO,
SAGAMI SEICHIRO
Publication year - 1993
Publication title -
international journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 93
eISSN - 1365-4632
pISSN - 0011-9059
DOI - 10.1111/j.1365-4362.1993.tb01400.x
Subject(s) - epidermis (zoology) , basal (medicine) , pathology , malignancy , carcinogenesis , biology , cellular differentiation , cytoplasm , basal cell epithelioma , cancer research , microbiology and biotechnology , basal cell , medicine , cancer , anatomy , endocrinology , biochemistry , genetics , gene , insulin
Background . Ras p21, a ras oncogene product, plays an important role in tumorigenesis, proliferation, and differentiation in various tissues and cells. Methods . Using a monocolonal antibody raised against ras p21 (RASK 4), localization of ras p21 in normal epidermis and involved epidermis of various skin diseases was examined immunohistologically. Results . Ras p21 was not present in basal cells of normal epidermis or basaloid cells of basal cell epithelioma but was found almost evenly in the cytoplasm of squamous cells and granular cells. This suggests that ras p21 is concerned with differentiation of epidermal cells. The mode of distribution of ras p21 differed from one cell to another in epidermal cells that turned to malignancy. The distribution was uneven and irregular in the tumorous region on the whole. Conclusions . This result might possibly represent abnormal differentiation of epidermal cells that turned to malignancy, deviating from the regular mode of the distribution of ras p21, which is necessary for normal differentiation.