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Tissue Level Lymphocyte Subpopulations in Donovanosis
Author(s) -
Sehgal Virendra N.,
Gupta Madan M.,
Jain Vinod K.
Publication year - 1991
Publication title -
international journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 93
eISSN - 1365-4632
pISSN - 0011-9059
DOI - 10.1111/j.1365-4362.1991.tb04351.x
Subject(s) - medicine , lymphocyte , immunology , lymphocyte subsets , immune system , t cell
Total T‐, B‐, T4‐, and T8‐lymphocyte populations were estimated in tissue sections of ulcers from 22 donovanosis patients, comprising 17 ulcerogranulomatous, four hypertrophic, and one sclerotic variants, using monoclonal antibodies and immunohistochemical technique (PAP). T‐ and B‐lymphocytic infiltrations in the tissues were almost identical, without any significant difference in ulcerogranulomatous and hypertrophic variants. The T4:T8 ratio in ulcerogranulomatous variants, however, was 1.41, which was significantly higher than that of hypertrophic variants (1.28), indicating a greater cell‐mediated immune response in the former than in the latter. This is substantiated by the documentation of a paucity of Donovan bodies, both in the tissue smear and slow (overnight) Giemsa‐stained tissue sections, in the ulcerogranulomatous variant.