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A Rapid Immunoperoxidase Technique to Distinguish Herpetic Types
Author(s) -
Ganderup Grace,
Newburger Amy E.,
Barr Ronald J.,
Riley Robert J.
Publication year - 1986
Publication title -
international journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 93
eISSN - 1365-4632
pISSN - 0011-9059
DOI - 10.1111/j.1365-4362.1986.tb03422.x
Subject(s) - immunoperoxidase , medicine , herpes simplex virus , staining , pathology , virus , virology , herpesvirus hominis , viral disease , immunology , herpesviridae , monoclonal antibody , antibody
The United States is experiencing an epidemic of herpetic infections. Rapid specific diagnosis of herpes simplex types And herpes zoster is important epidemiohgically and also for individual cases since treatment as well as prognosis for recurrence or dissemination varies with virus type. We report a rapid test for the diagnosis and differentiation of these infections. This test uses scrapings from viral vesicles. It employs standard immunoperoxidase techniques and takes 2 hours to perform at UC Smears from 1 3 patients with clinical and/or culture confirmation of viral type were tested. Eleven of eleven who had fresh smears (vesicles present less than 5 days) had positive staining for the viral type expected clinically. Two patients with vesicles 5 days or older showed nonspecific staining.