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Steroid‐induced Dermal Atrophy
Author(s) -
Booth Barbara A.,
Tan Elaine M. L.,
Oikarinen Aarne,
Uitto Jouni
Publication year - 1982
Publication title -
international journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.677
H-Index - 93
eISSN - 1365-4632
pISSN - 0011-9059
DOI - 10.1111/j.1365-4362.1982.tb03140.x
Subject(s) - collagenase , hydroxyproline , fibroblast , in vivo , atrophy , incubation , in vitro , microbial collagenase , medicine , proline , human skin , steroid , endocrinology , pathology , biochemistry , biology , enzyme , hormone , amino acid , microbiology and biotechnology , genetics
Collagen production by human skin fibroblast cultures was studied by incubation with [ 3 H] proline and several glucocorticosteroids known to produce dermal atrophy in vivo. Collagen production was measured as formation of [ 3 H]hydroxyproline or collagenase‐digestible 3 H‐polypeptides, and the values were corrected for changes in cell number in the same cultures. The steroids, in a wide concentration range, failed to elicit any consistent alterations in collagen production. Review of the literature dealing with corticosteroid‐induced changes in collagen production by human skin fibroblasts indicate conflicting results even under apparently similar incubation conditions. Consequently, no unifying hypothesis for steroid‐induced dermal atrophy can be developed presently based on the in vitro data.